Anaerobic Tubes and Culture Bottles

Hungate culture tubes

• Gas impermeable tubes for anaerobic culture conditions
• Sampling and additon of nutrients via syringes
• Consists of:
  • Culture tubes made of glass with round base and screw thread
  • Gas-impermeable, black butyl rubber stopper
  • Screw cap

Anaerobic culture tubes with aluminum caps (Balch type)

• Gas-impermeable tubes made of borosilicate glass for anaerobic culture conditions
• Particularly suitable for studies on bacteria that produce methane gas
• Gas-impermeable, blue septa made of butyl rubber

Anaerobic culture bottles

• Borosilicate glass type 1, class A
• Innovative 45 mm PP screw cap (GL45) with silicone O-ring and blue septum plug made of butyl rubber, designed to maintain anaerobic conditions
• Sizes: 100, 250, 500 and 1000 ml

DeLong® culture bottles

• Ideal for growing, storing and shaking aerobic and anaerobic cultures
• Borosilicate glass type 1, class A
• Graduation with approximate volume
• Available with stainless steel caps or KAP-UTS ™ or without caps

Crimping and unlocking pliers for anaerobic culture tubes and bottles

Caps and Closures

GL screw caps

• Polypropylene, inner sealing lip, autoclavable up to 140 °C, different colours
• PTFE/silicone liner,  32 mm and 45 mm
• Screwcap for high temperatures, PTFE/silicone liner, -196 °C to +260 °C, clear, USP Class VI, EP and FDA compliant
• Cap with 0.2 µm PTFE membrane, autoclavable
• PP Open top polypropylene caps for use with PFTE/silicone septa or access port assemblies
• Pouring rings for GL32 and GL45 available

KAP-UTS™ disposable closures

• For culture tubes and flasks with DeLong® style necks
• Manufactured from nontoxic polypropylene, autoclavable
• Seven colours and six sizes from 13 mm to 38 mm inner diameter
• Same evaporation and sterility characteristics as stainless steel closures
• Moulded pressure fins for firm grip
• Autoclavable up to 140 °C

Phenolic screw caps (bakelite) with liner

• Screw caps with white rubber or PTFE bonded liner
• Long cap design reduces risk of cross contamination
• Autoclavable (steam sterilization)

Silicone sponge closures

• Sealed-in silicone sponge filter is ideal for aerobic  cultures
• Flanged to fit snugly over outside of vessel necks
• Withstand dry heat sterilization up  to 180 °C

Silicone sponge plugs

• Tapered silicone sponge with smooth exterior for  tight seal
• Extra long for easy insertion and removal

Stainless steel closure

• For culture tubes and flasks with DeLong® style  necks
• Manufactured from 305 stainless steel
• With finger grips for firm fit
• Withstand dry heat sterilization and autoclaving

Cloning Cylinders

• For the isolation of single cells or clones from mixed populations on solid culture media (culture dishes)
• Four sizes available: 6, 8, 10 and 16 mm outer diameter
• Made from Borosilicate glass (or on request or in stainless steel)
• Suitable for heat sterilization and autoclaving

Cover Slips

Square and rectangular cover slips

•  Manufactured from Corning 0211 Glass
• Glass thickness: 0.13 mm - 0.16 mm
• Different sizes available

Round cover slips

• Manufactured from German Glass D 263M
• Glass thickness: 0.13 mm - 0.16 mm
• Diameter: 5 mm to 25 mm
• High chemical resistance
• Ideal for neuronal tissue cultures and less adherent cells
• Suitable for fluorescence and confocal microscopy

Photo-etched Cover Slips

Coding facilitates micromanipulation of cells or analysis of chromosome spreads

Round cover slips

• Manufactured from German Glass D 263M
• Coded area: 0.6 mm x 0.6 mm
• 100 (ø: 12 mm) or 200 (ø: 25 mm) alphanumeric coded squares
• Glass thickness: 0.13 mm - 0.16 mm

Square cover slips

• Manufactured from German Glass D 263M
• Coded area: 0.6 mm x 0.6 mm
• 520 alphanumeric coded squares
• Glass thickness: 0.19 mm - 0.25 mm

Erlenmeyer and Shake Flasks

Fernbach Flasks

• Ideal for bacterial cultures with high oxygen requirements
• Flask made of type 1 borosilicate glass, class A
• DeLong® neck with break-resistant lip, GL45 solid or membrane screw cap for fast pressure compensation (closed lid or ventilation lid)
• Without or with 3 bottom and/or 3 side baffles
• Capacity: 1800 ml or 2800 ml

Flasks and Bottles from Borosilicate glass, different styles

Biometer flasks

• To measure microbial CO2 production
• Delivery funnel to the flask for addition of e.g. acids and bases
• Second chamber to collect and harvest gas
• Made from Borosilicate glass type 1, class A
• White or brown glass for lightsensitive materials
• Autoclaveable

Hybridization Bottles Autoblot®

• Designed for DNA/RNA blotting procedures
• Type 1, class A Borosilicate glass
• Includes red PBT high temperature cap

Nephelo Culture Flasks

• DeLong® neck with stainless steal closures or phenolic rubber lined screw caps
• With or without cleanout port
• Also available with baffles for increased oxygenation

Filling Bell

• Aseptic filling bell from Borosilicate glass Type 1, class A
• Attaches to reservoir with rubber tubing
• Receiving vessel is placed under the bell
• Different sizes available for use with tubes or bottles
• Autoclavable

Homogenizators, BioVortexers, Tissue Grinders

A wide range of manual and semi-automatic homogenizers is available for a large number of applications in the field of disruption of tissues, cells and cell organelles.

Manually operated homogenizers (Glass)

• Dounce homogenizers
  • For cell disruptions
  • For extraction of cell nucleuses

• Conical shaped (Glass)
  • Ideal for disruption of heart, skeleton-muscle and lung tissue

• Potter-Elvehjem homogenizer
  • Entirely made of glass or with a pestle consisting of a stainless steel shaft and a PTFE-head
  • For disruption of tougher tissues, connective tissues (glass pestle) and soft tissues like liver or brain (PTFE-head)

• Tenbroeck homogenizer
  • With hollow glass pestle for cooling

• Cellector® Tissue Sieve
  • For the preparation of single cell suspensions and for gel extraction of proteins
  • Entirely made of stainless steel
  • Two dish sizes: 85 ml and 130 ml
  • Available in mesh sizes from 25 µm to 1910 µm

Semi-automated homogenizers

• BioVortexer
  • Batterie-powered homogenizer
  • Single use pestle for use with 0.5 ml  and 1.5 ml tubes

• Overhead drives

Microscope Slides

• Pre-cleaned microscope slides, 25 x 75 mm, plain or with one/two frosted ends
• Reich counting slides with three rings of 1 cm² interior area, for cell counting and calculation of bacterial sample cell concentration
• Cell counting slides for e.g. counting of somatic cells in cow’s milk. Spread 10 µl of sample onto each circle, dry, stain and count
• Fluorescent antibody slide with black ceramic background and 8 rings (6 mm diameter) for indirect fluorescent antibody tests

Pipette Containers

Pipette container from Borosilicate glass

• Round bottom or flat bottom with silicone pad
• PTFE cushion for round bottom containers available
• 38 mm diameter
• Different lengths available, also for „shortie“ pipettes
• Autoclavable and dry heat sterilisable
• Mounting plate to stabilize round containers

Pipette container from stainless steel

• Rectangular construction for easy stacking and storage
• Inside silicone pads at top and bottom
• Different sizes available
• Autoclavable and dry heat sterilisable up to 180 °C

Racks for test and culture

Leighton culture tube racks

• For 36 Leighton tubes
• 2 types available for tube lengths 83 - 93 mm and 125 - 150 mm

Roller culture tube rack for rotating culture tubes (roller tubes) / Hungate culture tubes

• Made of stainless steel
• For 64 positions
• Tube Ø max 16 mm

Test and culture tube racks made of stainless steel

• Rustproof and resistant in a water bath or in CO2 atmosphere
• Different sizes available for all common tube shapes

Roller Bottles

• Type 1, class A Borosilicate glass
• Diameter 11 cm to 12 cm for use with roller apparatus
• Different screw caps available:
  • 38 mm long skirted, black phenolic, rubber lined
  • 45 mm (GL45) PP caps
  • 51 mm flat, black phenolic, rubber lined
• Different lengths available
• Growth areas from 670 cm² to 1,585 cm²

Serological Glass Pipettes

• Type 1, class A Borosilicate glass
• Various styles available
• With or without cotton plugging top
• Blow out, tempered tip
• Calibrated to the tip
• Available: 3 mm orifice for handling tissue homogenates and cell suspensions
• "Shorties” for easy use under biological hoods
• Plug your pipettes with the suitable Pipette Plugger

Spinner Flasks and Bioreactor Vessels

Different types of spinner flasks and bioreactor vessels for various applications: suspension of bacteria, insect, mammalian or plant cells, as well as microcarrier culture. To be used with magnetic stirrers or overhead drives.

• Many sizes available: from 25 ml to 36 l
• Unjacketed and jacketed flasks
• Central neck: 70 mm or 100 mm
• Sidearm necks: GL32 or GL45
• Made from type 1, class A Borosilicate glass

Spinner flask types available

• Internal impeller assembly or height adjustable shaft
• With different types of impeller blades or magnetic stir bar only
• Spinner flasks with 2 or 4 sidearms
• Flat bottom or dimpled bottom to prevent cell accumulation
• MicroCarrier flask
• Internal impeller flask
• BELL-FLO™ flask with vertical baffles
• Glass Ball flask for gentle agitation
• Adjustable hanging bar flask
• BioProbe™ flask with 4 side arms
• Water-jacketed flasks

Bioreactor types available

• 4-45™ bioreactors with 4 GL45 sidearms
• Vertical Sidearm Bioreactor Vessels with 4 or 6 GL45 sidearms
• Lid with stainless steel connections (different variants and number of access ports) available
• Water-jacketed bioreactor vessels
• High or low profile vessel

Spinner Flask and Bioreactor Accessories

• Flasks Carriers
• Sparger and sparger assemblies
• Caps and impeller assemblies
• Probes and sensors (pH, oxygen, temperature)
• Shafts (for GL 45 caps)
• BioSimplex ™, sterile
• Various port assemblies
  • 2-Port assemblies
  • 3-Port assemblies with or without sparger
  • Thermowell assemblies
• Suitable open top caps and solid caps (please see above „Caps and Closures“)

Sykes-Moore Chamber

• For the study of cells by means of high magnification, phase optics, or time lapse techniques
• Cover slips are separated by a nontoxic silicone gasket
• The chamber has a 2.8 cm² viewing area and holds approx. 650 to 750 µl

Tubes

Centrifuge Tube

• Type 1, class A or B Borosilicate glass
• With 38 mm rubber lined phenolic screw cap
• Capacity: 590 ml or 780 ml
• Fits into I.E.C. No. 353A centrifuge adapters

Culture Tubes

• Type 1, class A or B Borosilicate glass
• Plain top to be used with KAP-UTS™ or stainless steel closures
• Different diameters and lengths
• Round bottom

Gas Fermentation Tube

• To be inverted in bottom of larger tubes for collection of fermentation gases
• Standard flint glass 1.5 mm thick
• Lenght 29 mm, Ø 8 mm

Leighton Tubes

• Type 1, class A Borosilicate glass
• Rectangular coverslips suitable for Leighton tubes available
• Short neck for easy positioning of cover slips
• Plain top or screw cap available
• Fits into Leighton tube rack (also available)